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The number of viable cells was measured by flow cytometry after addition of 5 l spike in fluorescently labeled bead (CountBright Plus Absolute Counting Beads, ThermoFisher Scientific) for normalization
The higher amount of affected processes detected in cardiac MTs may indicate increased susceptibility of cardiac MTs to the effects of DMSO, though there might also be a statistical bias due to different amount of DEGs used in the pathway analysis
Nature 619 (7969), 371377
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