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In zygotes formed by fertilizing normal eggs with sperm from hyperglycemic mice, insufficient TET3-mediated demethylation disrupted epigenetic reprogramming of sperm DNA, leading to hypermethylation of insulin secretion-related genes like Gck in the progenys pancreas

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Other materials and equipment required: Microplate reader with 450 nm wavelength filter Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips Incubator Deionized or distilled water Absorbent paper Buffer resevoir *Note: The below protocol is a sample protocol

Dangerous for multi-dose vials