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FNR, the master regulator involved in the adaptation to oxygen-limited conditions

Wound-healing assay NHEK (4 10 5 cells) were plated into 96-well plates and incubated in KBM2 supplemented with 0.4% bovine pituitary extract, 0.125 ng ml 1 rh EGF, 5 g ml 1 rh insulin, 0.33 g ml 1 hydrocortisone, 0.39 g ml 1 epinephrine, 10 g ml 1 transferrin and 0.06 mM CaCl 2 for 24 h, to allow the formation of a confluent monolayer

The aging process leads to reduced T-tubule density in cardiomyocytes, potentially facilitating post-depolarization phenomena, reentry events, and alternans, thereby establishing an electrophysiological basis for arrhythmia (Setterberg et al., 2021)