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The expression of target genes was analysed via quantitative real-time PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)

The increased MSTN expression was detected in in skeletal muscle of type 2 diabetic KKAy mice or palmitate treated C2C12 cells, while astragalus polysaccharide could downregulated the expression of MSTN and improve insulin sensitivity (65)

Furthermore, both Sll1545 and Slr0236 operate in the protection against stresses triggered by high light, H 2 O 2 , menadione and methylene blue

hypselobagri induces oxidative stress which results in the generation of reactive oxygen species in the host Wallago attu , with modulation in the level of essential antioxidant enzymes (GST, SOD, GPx, GR, CAT and LPO)