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Male C57BL/6 mice (78 weeks old, 2528 g) were purchased from the Institute of Laboratory Animal Science at the Chinese Academy of Medical Sciences (Beijing, China) and housed in a specific pathogen-free facility maintained at 2022 C, with cycles of 12 h light-dark and food and water ad libitum

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Intranasal delivery offers a direct route for systemic and neurological support, often used in protocols focused on wellness, cognitive clarity, and detoxification