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27 These conditions, including the antibodies used and the recombinant human Trx1 standards utilized, impacted accuracy of measurement and contributed to huge differences in Trx1 levels between studies, making between-study comparisons very difficult

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Complementary transcriptomic analyses reinforce this finding that genes involved in oxidative phosphorylation and mitochondrial metabolism are downregulated in PCOS granulosa cells compared to normal responders, supporting the hypothesis that defective bioenergetics and redox regulation underlie follicular insufficiency (58)