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At 80% confluence, cells were detached with 0.05% trypsinEDTA (Gibco) and seeded on membrane supports (12 mm Transwell culture inserts, 0.4 m pore size, Costar) coated with 0.05 mg collagen from calf skin (Sigma-Aldrich) in ready-to-use AEGM supplemented with 1% penicillin/streptomycin

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