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Bhattarai et al., 2021) and germ-free animals (Bhattarai et al., 2021)

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171 The levels of Wnt3, Wnt4, FZD 2, FZD 8, Wnt2b, Wnt5a, FZD3, LRP5, and sFRP3 are increased in the human spinal cord tissue of ALS patients

FIGURE 5 Sorted cells display elevated gene overexpression level We further demonstrated the cell sorting strategy in a gene overexpression experiment