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This explains why getting drunk may cause us to lose our coordination, react slowly, or forget things
The thousands of chemicals and the intense heat from burning tobacco directly irritate oral tissues and can cause chronic inflammation and damage to the salivary glands over time
The slices were soaked in physiological saline (three times for 5 min at room temperature), then incubated with a secondary antibody in 0.01% saponin and 0.1% BSA dissolved in physiological saline: goat-anti rat immunoglobulin G (IgG) (H+L) cross-absorbed, Alexa Fluor 594 (1:500, Invitrogen, A11007), or goat-anti chicken immunoglobulin Y (IgY) H&L (Alexa Fluor 488) (1:1,000, Abcam, ab150173) for 2 h at room temperature (protected from light)
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