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Cyclization: 1) preparation of cyclization reagent: dissolving iodine per gram in 30-40mL ethanol 2) Preparation of Vc reagent: dissolving Vc per gram in 20-30mL of purified water 3) Cyclization: the linear crude peptide was dissolved in 20% acetonitrile aqueous solution (1g of crude linear peptide in 100mL of 50% acetonitrile aqueous solution), and the linear peptide was dissolved and diluted to 1g/L with purified water
The cost calculation is straightforward: a $3 vial of bacteriostatic water saves hundreds of dollars in peptide waste
The tripeptide fragment, however, exhibited only about 40% of the potency of [D-Arg]dermorphin and showed an inhibitory effect roughly equivalent to that of morphine
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