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Since the current study collected serum on day 1, this may indicate that the increase in TCA occurs early and then cysteine is utilized for glutathione synthesis rather than for taurine biosynthesis, which results in decreased concentrations of the taurine conjugates

J Biosci Bioeng 129:5258

Cells were harvested with trypsin digestion, pelleted, and washed with PBS before being analyzed by flow cytometry (BD Accuri C6)

The Doa1-Cdc48 -Ufd1-Npl4 complex formed by the Ufd1-Npl4 heterodimer and Cdc48 strips ubiquitinated proteins from the mitochondrial surface via the AAA-ATPase function of Cdc48 and delivers them to the proteasome for degradation [50]