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In both pathways, cysteine-dependent proteases known as caspases execute apoptotic cell death by cleaving key structural and regulatory proteins, primarily through the action of caspase-3 (CASP3) and caspase-7 (CASP7), with caspase-6 (CASP6) contributing to downstream substrate cleavage and apoptotic progression

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Before and 30, 60, and 120 min after the administration, the rats were sacrificed by puncturing the inferior vena cava under a pentobarbital sodium (4050 mg/kg) anesthesia