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First, when you take a pill, it has to be digested and metabolized through the liver or kidney through what is called the first-pass effect

We then built the isobologram of the corresponding dose-response curves with a fixed dose ratio of R and compared the expected mixture of the two doses that produced an effect equal to half of the maximal effect produced by the individual drugs and to the experimental value (see later in methods)

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O (Outcome): Primary outcome: changes in serum Vitamin B12 levels, clinical improvement of Vitamin B12 deficiency symptoms