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After we incubated compound 2 ( t R = 19.8 min) with the CLs supplemented with dithiothreitol (DTT) for 1 min at 37 C, two new peaks corresponding to compound 3 ( t R = 20.2 min) and compound 1 ( t R = 21.2 min) appeared (Fig

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The combination limited and variable BBB transport plus rapid plasma degradation means peripherally administered DSIP delivers a brief and structurally heterogeneous exposure to the central nervous system, complicating dose-response interpretation in any whole-animal experiment