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In parallel with an increase in GSTA1 and a decrease in TG level after transfection with the GSTA1 plasmid in OA-treated L02 cells, gene expression involved in the esterification of FA to TG (endoplasmic reticulum-bound stearoyl-CoA desaturase 1 ( SCD1 ), glycerol-3-phosphate acyltransferase 3 ( GPAT3 ), LIPIN and diacylglycerol acyltransferase 1/2 ( DGAT1 , DGAT2 ), FA de novo lipogenesis (acetyl-coenzyme A synthetase 2/3 ( ACSS2 , ACSS3 ), sterol response element-binding protein 1 ( SREBP1 ) and fatty acid synthase ( FAS )), and FA -oxidation (carnitine palmitoyltransferase 1 alpha ( CPT1A ), acyl Co-A oxidase ( ACOX1 ) and acetyl Co-A carboxylase 2 ( ACC2 )) were decreased in a dose-dependent manner (Figure 6CE)
Increased FAO in cytochromes may then be an important event in steatosis and NASH, with the excessive amount of ROS produced by the CYP enzymes exacerbating hepatic oxidative stress and consequently worsening liver damage
