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It decreases LPS-induced increases in the levels of reactive oxygen species (ROS), IL-6, and TNF- in RAW 264.7 cells when used at a concentration of 10 M.3 GHK-Cu (10 g/g) prevents LPS-induced decreases in lung superoxide dismutase (SOD) activity and glutathione (GSH) levels and reduces LPS-induced increases in the number of cells and the level of total protein in bronchoalveolar lavage fluid (BALF) in a mouse model of acute lung injury.

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GSH scavenges a wide range of ROS ( OH, H 2 O 2 , 1 O 2 ), and due to the very low value of electron reduction potential of the GSSG/GSH redox couple ( E = 264 mV at pH = 7.4) is GSH able to regenerate several important antioxidants, such as radicals of vitamins E and C back to their active non-radical forms (Jones 2002) (Fig