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Increased permeability of the gut barrier has been observed to occur with a variety of physiological stressors including heat stress [4, 5], exercise stress [6], psychological stress [7], excessive dietary fat intake [8], lack of fibre [9], and sleep deprivation [10] many of these are also risk factors for hypertension [11, 12]

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Fully automated two-step assay for detection of metallothionein through magnetic isolation using functionalized -Fe2O3 particles