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Organoids were passaged every 57 days by harvesting in 12 mL of ice-cold Advanced DMEM/F12++ (Advanced DMEM/F12 containing 10 mM HEPES and 1 GlutaMAX) per 600 L of Cultrex or Matrigel, centrifuging at 400 g for 5 min at 4 C, resuspending in 1 mL ice-cold Advanced DMEM/F12++, and triturating 15 times with a 1 mL pipette, followed by 30 times with a P10 tip attached to the end of the P1000 tip

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Monavari AA, Naughten ER: Prevention of cerebral palsy in glutaric aciduria type 1 by dietary management

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