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Mature, well-differentiated monolayers of cells were then exposed to control (ultrapure water dissolved in culture medium, water/medium: 2% v/v) or e-cig smoke solution (containing 2 ppm diacetyl, water/medium: 2% v/v) on the apical side for 6 or 24 h (n = 3 subjects, each treatment was performed in duplicate)

(Jacksons reaction to losing out to Martin Landau in the Supporting Actor category a clearly visible Shit! has since entered Oscar folklore.) No matter though: by the end of the year, Pulp had become an international phenomenon, its influence felt in music and advertising, television and literature

Six years after two-year-old Aldi started sneaking ciggies while his mother sold fish at the market, Fairfax Media tracked him down in the remote village of Teluk Kemang in South Sumatra

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