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To confirm that human TfR1 is present in the Affi-Gel 15-isolated proteins, including human TfR1 as known from the initial MS analyses, immunoblotting was carried out using anti-TfR1 (Abcam, Cambridge, MA, ab-84036, 1 g/mL) and an HRP-linked second antibody (1:5,000 polyclonal rabbit anti-IgG, 656120, Thermo Fisher Scientific) using whole-cell extracts (1520 g) on 8% or 4%20% SDS-PAGE gels, followed by transfer to PVDF membranes

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The primary endpoint was HbA1c change from baseline to 24 weeks, and secondary endpoints included change in HbA1c and bodyweight at 36 weeks

What the broader literature describes is remodeling, not a one-way pile-up