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In a randomized, placebo-controlled intervention study with more than 900 older participants experiencing psychological distress, daily supplementation with folic acid (400 g) and vitamin B 12 (100 g) for two years did not reduce the occurrence of symptoms of depression despite significantly improving blood folate, vitamin B 12 , and homocysteine levels compared to placebo (109)

By using GHK-Cu as a single, well-characterized entity, researchers can evaluate both localized and systemic effects of copper peptide signaling without the confounding variability of mixed or poorly defined copper complexes