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Examples of potential inclusion criteria, if this approach were taken, are i) a score greater than 4 in the 12-question TSQ, a validated instrument used to assess hypothyroid symptomatology, or ii) an overall health-related QOL score using the ThyPRO-39 composite score of more than 32 (167)

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Next, the supernatant was collected after centrifuging at 1000 rpm for 10 min at 4 C, and then measured at 532 nm with a microplate reader