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ProtocolRat Female Sprague-Dawley rats (n=6-8 per group) weighing 200-300 g and of approximately 6-8 weeks of age served as subjects for the experiments

Intranasal administration of peptides has been shown effective due to the following factors [27]: Large surface area Permeable endothelial membrane Elimination of first-pass effect Good circulation Researchers who wish to administer the BPC-157 and TB-500 blend via a nasal spray are encouraged to take certain precautions to ensure delivery of maximum bioavailability: To optimize absorption: Wait up to one minute between sprays via the same nostril to prevent oversaturating the nasal membrane and thus losing peptide due to swallowing

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