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DE was induced using the STEMdiff TM Definitive Endoderm Kit (Stem Cell Technologies)

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Quantitative real-time PCR RT-PCR was performed using the Taqman real-time PCR assay (Applied Biosystem, USA) for the following molecules: Hprt (Mm00446968_m1), Il-1 (Mm00434228_m1), Myd88 (Mm00440338_m1), Xdh (Mm00442110_m1), Il18 (Mm00434226_m1), Il-33 (Mm00505403_m1), Type 1 collagen (Mm00801666_g1), inos (Mm00440502_m1) and Nlrp3 (Mm00840904_m1)

The peptide's effects on the gastrointestinal tract involve interaction with the nitric oxide system, providing endothelium protection and angiogenic effects even in severely compromised conditions