Unless stated otherwise, the following concentrations were applied: 5 M erastin, 1.13 M RSL3, 10 M FIN56, 10 M FINO2, 10 M sorafenib, 1 M Fer-1, 50 M DFO, and 1 M dexamethasone
Neuron 109 , 34563472.e8 (2021)
Figure 5 While compelling evidence establishes ferroptosis as a selective vulnerability of RCC and validates its potential for therapeutic exploitationvia natural compounds, reversal of drug resistance, and combination regimensseveral critical, insufficiently addressed research gaps persist
Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C