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10 Discussion Cancer is the leading cause of death and immunotherapy has attracted a wide range of attention due to its advantages such as durable response, long-term survival benefit, and lower toxicity and side effects (242, 243), in which immune cells are the target cells for immunotherapy, which as part of the TME are mainly composed of TAMs, DCs, NK cells, TANs, MDSCs and T cells

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Results Nestin expressing cells preferentially differentiate into osteoblast lineage cells postnatally Cells expressing Nestin have previously been reported to represent a subset of bone marrow mesenchymal stem cells as they differentiate in vivo into multiple lineages, including osteoblasts, chondrocytes, and adipocytes (27)

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