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Changes of less than 30% were deemed mild, those between 30% and 50% were deemed moderate, and those beyond 50% were deemed severe ( 2.11 Immunohistochemical evaluation Liver tissue sections were deparaffinized in xylene and then rehydrated in graded alcohols ( 2.11.1 Assessment of SIRT1 and NF-kB p65 immunostaining Five tissue slices were evaluated for SIRT1 and NF-kB p65 quantitative immunoreactivity in each group

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Desmet J, van Dael H, van Cauwelaert F, Nitta K, Sugai S: Comparison of the binding of Ca 2+ and Mn 2+ to bovine -lactalbumin and equine lysozyme