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A randomized controlled trial in 126 pregnant women found that continuing folic acid supplementation of 400 g/day beyond the first trimester of pregnancy (i.e., gestational weeks 14 throughout the second and third trimesters) did not increase blood concentrations of unmetabolized folic acid at 36 weeks gestation compared to placebo despite increases in plasma total folate, plasma 5-MTHF, and red blood cell folate (207)