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Since the Raman spectra are dominated by the amide bands of the peptide backbone, similar to ORD and CD, it can be used for the analysis of protein secondary structure by finding correlations between the positions of the amide I and amide III vibrations and fraction of each secondary structural element present in globular proteins determined from X-ray crystal structures (Williams, 1986

11, Oct

This is part of why peptide-focused vendors emphasize per-puncture aseptic technique and dedicated needles per draw direction

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