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MilliporeSigma) in the presence of 10 M amiloride under a basolateral-to-apical K + gradient and an apical-to-basolateral Na + gradient as previously described 24

The circuits involved in addiction are the: amygdala and hippocampus affected by fear, nucleus accumbens and the ventral pallidum which respond to rewards that increase dopamine, anterior cingulate gyrus and prefrontal cortex, responsible for cognitive control, downregulate control of the orbito-frontal cortex, and Orbito-frontal cortex evaluates the value of rewards and the decision to use a substance is given

Furthermore, the two pieces of wood that hold up the tray are going to take up some space, reducing the capacity slightly