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The cell pellets were further fixed for 24 h at 4 C and post-fixed for 1 h using a reduced osmium solution prepared by combining 3% potassium ferricyanide with an equal volume of 4% aqueous osmium tetroxide in 0.3 M cacodylate buffer

VPA-Induced ASD Model Group In the ASD model, significant genus-level alterations in the gut microbiota were observed (Figure S2A)