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Rather, it is a nervous system disorder that may have accompanying mental health concerns, such as anxiety and depression

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found that HIF-1 was positive in muscle biopsy samples of juvenile DM (jDM, DM patients under 16 years old), accompanied by significantly elevated HIF-1 expression levels as indicated by mRNA data

Methods Antibodies Anti-MBP tag mouse monoclonal antibody and anti c-Jun rabbit monoclonal antibody was used at 1:1000 dilution (Beyotime, AF2912, AF1612), anti-ubiquitin was used at 1:20000 dilution (Abcam, ab134953), anti-GST tag mouse monoclonal antibody was used at 1:5000 dilution (Yeason, 30901ES50), HRP-conjuagated FLAG-tag mouse monoclonal antibody was used at 1:1000 dilution (Yeason, 30502ES60)