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the animals were allowed to recover from surgery for several days before use in any experiment, and were fasted overnight prior to the the catheter was flushed with saline solution and a volume of saline equal to the volume of blood taken was injected (to maintain total blood volume)

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In the case of skin necrosis, two types are distinguished: dry gangrene, which is not caused by bacteria, and wet gangrene, which develops due to bacterial proliferation near the wound

Clin Kidney J 8(2):180187 Fitzgibbon G, Mills KH (2020) The microbiota and immune-mediated diseases: opportunities for therapeutic intervention