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Frozen storage at minus twenty degrees Celsius The minus twenty degree freezer is the standard recommendation for long-term peptide storage

The antioxidant solution was prepared as follows: a stock solution containing 4% ascorbic acid (VWR, Leuven, Belgium), 0.4% glutathione and 2% uric acid (Sigma-Aldrich, Lyon, France) was prepared in distilled water and filtered using a 0.2 m filter after adjusting the pH to 7.5 with 10 M KOH

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In combination with reactivation of mutant p53 by APR-246, the oxidation of Trx might facilitate p53-dependent apoptosis