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Experimental validation Dose response curves and determination of IC 50 values were assessed for the 12 candidate drugs using 3-fold dilution series (see Supplementary Methods (Supplementary File 1) for more details)

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Bacteriostatic water (BW) sterile water containing 0.9% benzyl alcohol as a preservative is the standard medium for peptide reconstitution because: Benzyl alcohol inhibits bacterial contamination without degrading the peptide It maintains stability for extended storage periods after reconstitution It is isotonic, minimizing osmotic disruption in cell culture systems Key Research Facts and Statistics: Peptide Sciences in Numbers The Role of Peptide Sciences in Longevity and Anti-Aging Research One of the most compelling frontiers in peptide sciences is its application to geroscience the study of aging biology