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These purified HBs demonstrated robust self-renewal capacity, expanding in a small-molecule-defined medium (ACDFSV) for over 20 passages without losing their proliferative capacity or characteristic morphology

However, bridging the gap to human clinical application is where the "science" gets complicated

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qPCR was performed with SYBR Green Supermix (TaKaRa) with the protocol as follow: 95 C for 5 min, repeating 40 cycles of 95 C for 5 s and 60 C for 45 s using StepOnePlus real-time PCR system (Applied Biosystem, Foster City, CA, USA)