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The study found that triglycerides cross the blood-brain barrier and can induce leptin resistance.[ref open access] Theoretically, it makes sense that high triglycerides would block the leptin signal in times of starvation, driving people to need more food

Stomach acid denatures protein structures

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The MRP2 expression vector pcDNA5/FRT-MRP2 was constructed by cloning the ABCC2 gene into the eukaryotic expression vector pcDNA5/FRT (Life Technologies) as described previously 59 with an additional Kozak sequence added at the beginning of the gene