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The thigh, buttocks, or upper arm are the best spots due to their muscle depth and ease of access

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A 25% Poloxamer 407 stock solution in PBS buffer and Poloxamer 188NF from BASF are used

Furthermore, the relative abundance of Akkermansia , a genus positively associated with improvements in obesity-related metabolic issues 60 , was increased in the vehicle-treated group that underwent diet switch relative to the WD control group, suggesting a potential for distinct microbiome-mediated mechanisms of weight loss subsequent to diet switch from those of diet switch in combination with 5A-1MQ treatment