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Description

A Superdex 200 increase 5/150 GL column (GE Healthcare) was calibrated with protein standards (molecular weight marker kit MWGF200 and thyroglobulin and apoferritin from Sigma) in the presence of 20 mM HEPES-NaOH pH 7.5, 150 mM NaCl at a flow rate of 0.45 mL min 1

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