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The latter regulates the Nrf2-ARE pathway mainly through phosphorylation sites and is considered a critical regulatory factor of Nrf2 nuclear accumulation, nuclear rejection, and degradation

With regard to AG-120 experiments, cells were first cultured with low MgSO4 media (0.08 mM) for 24 hours, followed by AG-120 treatment under 2 mM glutamine, 5% FBS and indicated glucose levels for 72 hours

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