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Microscale thermophoresis (MST) MST experiments were carried out on a Monolith NT.115 instrument (NanoTemper)

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After 72 h of compound incubation, cells were washed with PBS and 10% MTT solution (Abcam, Cat# ab146345) was added in phenol red-free medium for 3 h at 37 C

On this basis, those affected can try to eat a low-histamine diet for three to four weeks , i.e