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Once in the portal circulation, the copper complexes to albumin or amino acids with only a small fraction remaining free. A copper transporter (hCTR) for albumin-bound copper then transports the copper into the hepatocyte.12,13 There it interacts with several proteins that direct the copper to chaperones for incorporation into essential proteins (such as ceruloplasmin, copper/zinc superoxide dismutase, and cytochrome c oxidase), or into lysosomes for biliary excretion

The tissue was then homogenized in 10 mL PBS for five cycles at 3000 rpm using a PT 3100 polytron homogenizer

Bleeding can occur for up to 45 minutes without halting, so do not be too worried it happens for that long

In some embodiments, the formulation or composition has a viscosity of about 80 cP