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Giles, FJ, Andreeff M , Keating MJ

in: PubMed | Google Scholar | 1 The First Affiliated Hospital of Jinan University, Guangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of the Chinese Ministry of Education, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, and The Sixth Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China

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Using Pt GSTF8 from poplar as a model enzyme, we use biochemical, structural and computational methods to demonstrate that arGSTs employ a GSH-dependent Brnsted base mechanism for conversion of the LDOX product flavan-3,3,4-triol ( 4 ) or flavan-3-on-4-ol ( 7 ) into flav-2-en-3,4-diol ( 8-B4 ), an equilibrium form of cyanidin