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It has been shown that the redox potentials of roGFP variants, roGFP3 and roGFP4, which both contain the engineered disulfide C149/C202, are more negative than the redox potentials of their respective counterparts roGFP1 and roGFP2, which both contain the disulfide C147/C204 (Hanson et al., 2004)

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Zinc (Zn) and selenium (Se) levels were estimated by atomic absorption spectrophotometry