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Optical density of the obtained supernatant was read at 532 nm and level of mtLPO was expressed as nmol MDA/mg protein (molar extinction coefficient 1.56 105 M 1 cm 1 )

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Based on these results, we propose that inhibition of RNR (or upstream enzymes) leads to accumulation of GSH which can be re-purposed for use by GPX4 to prolong the inhibition of ferroptosis under conditions where new GSH synthesis is otherwise impossible (Fig 6)

It produces two primary hormones, T4 (thyroxine) and T3 (triiodothyronine), which regulate how quickly cells convert oxygen and calories into energy