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The cells were differentiated with PC-conditioned medium and cARLA (48 h) and the confluent monolayers were incubated with nanocarriers (100 g/ml) diluted in hEC culture medium at 37 C for 24 h in a CO 2 incubator

However, the exact time frame depends on the type and preservatives in the solution

The primary SELENOP/LRP8-dependent mechanism of Se entering the brain is rather well described

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