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The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea

As always, its important to talk to your healthcare professional prior to engaging in any peptide-related therapy

Through sacrificial oxidation, 7-DHC produces protective derivatives, such as 3,5-dihydroxycholest-7-en-6-one (DHCEO), which disrupt lipid peroxidation chain reactions and mitigate ferroptosis (Freitas et al., 2024

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