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Essential mineral nutrition in plants: integrated physiological, transcriptional, and hormonal mechanisms Essential nutrients are fundamental for plant growth and development, participating directly and indirectly in key metabolic processes, including photosynthesis, respiration, carbon and nitrogen assimilation, protein synthesis, nucleic acid formation, and enzymatic regulation (Anjum et al

For complementary DNA (cDNA) synthesis, the MMLV reverse-transcriptase kit (Invitrogen, Life Technologies, Carlsbad, CA) was used according to the manufacturers instructions

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