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Direct application of a variety of E-liquids to human Saos-2 and MG-63 cells, (another osteoblast-like cell line, derived from a human osteosarcoma) at concentrations up to 4%, delivering a nicotine dose of up to 1 mg/ml (falling within a theoretical physiological exposure range of 1-3 mg of nicotine per cigarette) reduced cellular viability, independent of nicotine [41]