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The nose-only method of forced smoke inhalation is a contentious research method used by a research group at the Centenary Institute, Sydney, in which mice are constrained in a tube and attached to a smoking machine, where they are forced to smoke up to 12 cigarettes twice a day for up to 18 weeks before being killed to measure disease indicators

For example, the breakdown of nicotine and its metabolites might slow down the breakdown of testosterone in the body, which could have the effect of increasing testosterone levels in the blood

The equipment was donated to the Institute for In Vitro Sciences (IIVS), a non-profit laboratory in Gaithersburg that carries out animal-free testing

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